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JoVE Journal
Neuroscience

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Subcellular Patch-clamp Recordings from the Somatodendritic Domain of Nigral Dopamine Neurons
 

Subcellular Patch-clamp Recordings from the Somatodendritic Domain of Nigral Dopamine Neurons

Article DOI: 10.3791/54601-v 09:17 min November 2nd, 2016
November 2nd, 2016

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Summary

Subcellular patch-clamp recordings offer the possibility to investigate the functional properties of dendrites. However these fine structures are not easily accessible due to their small diameter. The protocol described here aims to facilitate the collection of stable and reliable recordings from the dendrites of dopamine neurons in slices in vitro.

Tags

Keywords: Subcellular Patch-clamp Dopamine Neurons Somatodendritic Domain Ion Channels Neurobiology Brain Slices Coronal Sectioning Substantia Nigra Cell-attached Recording Gigaohm Seal
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