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人肝细胞通过一个两步胶原酶灌注过程隔离
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JoVE Journal Biology
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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人肝细胞通过一个两步胶原酶灌注过程隔离

DOI:
10.3791/50615-v

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07:09 min

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September 03, 2013

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, , , ,
1Experimental Surgical Research,Grosshadern Hospital, Munich, 2Center for Liver Cell Research,Grosshadern Hospital, Munich, 3Hepacult LLC, Regensburg, 4Department of Surgery,Grosshadern Hospital, Munich

Chapters

  • 00:05Title
  • 01:26Preparation of Perfusion Equipment and Solutions
  • 02:00Perfusion of the Liver
  • 04:29Isolation of Hepatocytes
  • 06:03Isolated Hepatocyte Viability, Yield and Purity
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

对于人肝细胞的分离的修饰的两步胶原酶灌注过程进行说明。此方法也可应用到其它哺乳动物的肝脏。孤立的肝细胞有很高的产量和活力提供，使他们在诸如肝再生，药代动力学和毒理学领域一个合适的模型进行科学研究。

Tags

Keywords: HepatocytesLiverCollagenase PerfusionIsolationTwo-stepHumanRegenerationPharmacokineticsToxicologyTranslational ResearchEnzymatic MethodStructural IntegrityFunction

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