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Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
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Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model

DOI:
10.3791/50670-v

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12:37 min

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September 07, 2013

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, , ,
1The Markey Cancer Center,University of Kentucky College of Medicine, 2Graduate Center for Toxicology,University of Kentucky College of Medicine, 3Department of Molecular and Biomedical Pharmacology,University of Kentucky College of Medicine, 4Department of Pediatrics,University of Kentucky College of Medicine

Chapters

  • 00:05Title
  • 02:12Preparation of C57Bl/6 K14-Scf Mice and Topical Forskolin Treatment
  • 04:31Reflective Colorimetry and Calculating the Minimal Erythematous Dose
  • 08:15Representative Melanin Induction Results
  • 11:43Conclusion

Summary

Automatic Translation

Automatic Translation

Epidermal melanin is induced by topical application of forskolin in a murine model of the fair-skinned UV-sensitive human. Pharmacologic manipulation of cAMP levels in the skin and epidermal darkening strongly protect against UV-mediated inflammation (sunburn) as measured by the minimum erythematous dose (MED) assay.

Tags

Keywords: Melanocortin 1 ReceptorMc1rStem Cell FactorScfEpidermal MelaninUV ProtectionForskolinMinimal Erythematous DoseMEDHumanized Mouse Model

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