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Journal / Immunology and Infection / Novel Whole-tissue Quantitative Assay of Nitric…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response
 
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Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response

Article DOI: 10.3791/50892-v • 07:49 min • December 4th, 2013
December 4th, 2013 •
1, 1
1Department of Biological Sciences, University of Alabama

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Levels of the inflammatory cell-signaling molecule nitric oxide (NO) are commonly assayed using Griess reagent. In this protocol, we have created a modified Griess assay utilizing live Drosophila brain tissue in order to detect the secretion of NO in a simple, quantifiable and highly repeatable method.

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Keywords: Nitric Oxide Neuroinflammation Drosophila Parkinson's Disease Griess Reagent Neurotoxin Innate Immune Response Cell-signaling Neurodegenerative Disorders Whole-tissue Assay
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