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Analisi di apoptosi in embrioni di zebrafish da Whole-mount immunofluorescenza per rilevare caspasi 3 attivata
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Biology
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JoVE Journal Biology
Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
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Analisi di apoptosi in embrioni di zebrafish da Whole-mount immunofluorescenza per rilevare caspasi 3 attivata

DOI:
10.3791/51060-v

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08:20 min

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December 20, 2013

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, , ,
1Department of Oncological Sciences,University of Utah

Chapters

  • 00:05Title
  • 01:24Preparation of Solutions and Embryos
  • 03:17Immunofluorescence Staining and Microscopy
  • 05:43Representative Results
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

Tags

Keywords: ZebrafishEmbryosApoptosisWhole-mount ImmunofluorescenceActivated Caspase 3TUNEL AssayIntrinsic ApoptosisExtrinsic ApoptosisCell TypesFixed EmbryosMutantsMorphantsOverexpressionChemicalsAcridine OrangeCost-effectiveReliable

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