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Laser nanosurgery af cerebellare Axoner<em> In Vivo</em
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JoVE Journal Neuroscience
Laser Nanosurgery of Cerebellar Axons In Vivo

Laser nanosurgery af cerebellare Axoner<em> In Vivo</em

DOI:
10.3791/51371-v

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09:25 min

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July 28, 2014

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, ,
1European Laboratory for Non-Linear Spectroscopy,University of Florence, 2National Institute of Optics,National Research Council, 3Department of Physics and Astronomy,University of Florence, 4International Center for Computational Neurophotonics (ICON Foundation)

Chapters

  • 00:05Title
  • 02:02Axonal Labeling
  • 04:42Optical Window on the Cerebellar Cortex
  • 06:29In Vivo Multi-photon Laser Axotomy
  • 07:56Results: Laser Nanosurgery of Cerebllar Axons In Vivo
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

To-foton billeddannelse, koblet til laser nanodissection, er nyttige værktøjer til at studere degenerative og regenerative processer i centralnervesystemet med subcellulær opløsning. Denne protokol viser, hvordan til at mærke, billede, og dissekere enkelt klatring fibre i den cerebellare cortex in vivo.

Tags

Keywords: Laser NanosurgeryCerebellar AxonsIn VivoTwo-photon Fluorescence ImagingNeuronal RegenerationClimbing FibersOlivocerebellar NeuronsAnterograde TracingDextran-conjugated DyeCranial WindowTi:Sapphire Laser

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