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Neuroscience
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谱系重编程的成人大脑的周细胞源性细胞诱导成神经细胞
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
Please note that all translations are automatically generated.
Click here for the English version.
谱系重编程的成人大脑的周细胞源性细胞诱导成神经细胞
DOI:
10.3791/51433-v
•
09:36 min
•
May 12, 2014
•
Marisa Karow
,
Christian Schichor
,
Ruth Beckervordersandforth
,
Benedikt Berninger
4
1
Department of Physiological Genomics, Institute of Physiology
,
Ludwig Maximilians University Munich
,
2
Tumor Biology Lab, Neurosurgical Clinic
,
Ludwig-Maximilians University Munich
,
3
Institut für Biochemie, Emil-Fischer-Zentrum
,
Friedrich-Alexander-Universität Erlangen-Nürnberg
,
4
Institute of Physiological Chemistry and Focus Program Translational Neuroscience
,
Johannes Gutenberg University Mainz
Chapters
00:05
Title
01:34
Isolation and Culturing of Adult Human Brain Cells
02:55
In vitro
Expansion and Characterization of Adult Human Brain Cells
05:15
Retroviral Transduction of Pericyte-derived Cells and Reprogramming into Induced Neurons
06:41
Results: Reprogramming Adult Brain Cells into Induced Neurons
09:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
针对脑固有细胞直接谱系重编程提供了脑修复的新观点。在这里,我们描述了如何准备丰富的文化从成人大脑皮质脑居民周细胞,并通过转录逆转录病毒介导因子的表达Sox2的和ASCL1将这些转换引起的神经元的协议。
Tags
Lineage-reprogramming
Pericyte
Adult Human Brain
Induced Neurons
Direct Conversion
Neurogenesis
In Vitro Modeling
Brain Repair
Cerebral Cortex
PDGF Receptor-β
CD146
Sox2
Ascl1
Pericyte-derived Induced Neurons
Patch-clamp Recording
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