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谱系重编程的成人大脑的周细胞源性细胞诱导成神经细胞
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
DOI:

09:36 min

May 12, 2014

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Chapters

  • 00:05Title
  • 01:34Isolation and Culturing of Adult Human Brain Cells
  • 02:55In vitro Expansion and Characterization of Adult Human Brain Cells
  • 05:15Retroviral Transduction of Pericyte-derived Cells and Reprogramming into Induced Neurons
  • 06:41Results: Reprogramming Adult Brain Cells into Induced Neurons
  • 09:11Conclusion

Summary

Automatic Translation

针对脑固有细胞直接谱系重编程提供了脑修复的新观点。在这里,我们描述了如何准备丰富的文化从成人大脑皮质脑居民周细胞,并通过转录逆转录病毒介导因子的表达Sox2的和ASCL1将这些转换引起的神经元的协议。

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