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Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
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Neuroscience
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JoVE Journal Neuroscience
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay

DOI:
10.3791/51865-v

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10:14 min

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September 02, 2014

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, ,
1The Shraga Segal Department of Immunology, Microbiology & Genetics, Faculty of Health Sciences,Ben-Gurion University of the Negev

Chapters

  • 00:05Title
  • 01:35Mouse Brain Protein Extraction
  • 03:25Telomeric Repeat Amplification Protocol (TRAP)
  • 06:12Agarose Mini-gel Electrophoresis and Gel Staining
  • 08:16Results: The Modified TRAP Assay is More Sensitive than the Traditional Assay in Detecting Telomerase Activity
  • 09:45Conclusion

Summary

Automatic Translation

Automatic Translation

Telomerase is expressed in the neonatal brain and also in distinct regions of the adult brain. We present a non-toxic time saving TRAP assay for the analysis of telomerase activity in various regions of the mouse brain and detection of differences in telomerase activity between male and female mouse brains.

Tags

Keywords: Telomerase ActivityMouse BrainNon-radioactive TRAP AssayTelomere LengthGenomic IntegrityNeuronal ProtectionAge-dependentGender Differences

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