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बृहतभक्षककोशिका Phagocytosis और actin polymerization के आकलन के लिए उच्च Throughput Fluorometric तकनीक
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
DOI:

09:22 min

November 27, 2014

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Chapters

  • 00:05Title
  • 01:38Plating and Treating Cells
  • 02:16Opsonization of Flourescent Particles
  • 03:36Phagocytosis
  • 05:08Actin Staining
  • 06:10Quantification
  • 07:33Results: Confocal Microscopy of Phagocytosis and Actin Polymerization
  • 08:47Conclusion

Summary

Automatic Translation

Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

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