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样品制备策略的3D细胞培养模型质谱成像
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
DOI:

08:14 min

December 05, 2014

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Chapters

  • 00:05Title
  • 01:28Three-dimensional Cell Culture and Preparation of Agarose Coated 96 Well Plates
  • 02:32Seed and Harvest the Three-dimensional Cultures and Embed in Gelatin
  • 04:11Slice and Thaw Mount the Gelatin-embedded Three-dimensional Cell Cultures for Mass Spectrometric Imaging
  • 05:03Matrix Application and Sample Preparation for MALDI Imaging
  • 05:42MALDI-MSI Analysis Using a MALDI-TOF System and Optional Data Analysis Methods
  • 07:28Results: Mass Spectrometry Imaging of Three-dimensional Cell Cultures
  • 07:49Conclusion

Summary

Automatic Translation

永生化癌细胞系可以种植三维细胞培养物,生物研究的一个有价值的模型。本协议描述的3D细胞培养,包括改进的样品制备平台质谱成像。该协议的目的是为了指导用户以制备三维细胞培养物用于质谱成像分析。

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