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Prøve Forberedelse Strategier for massespektrometri Imaging af 3D cellekulturmodeller
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Prøve Forberedelse Strategier for massespektrometri Imaging af 3D cellekulturmodeller

DOI:
10.3791/52313-v

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08:14 min

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December 05, 2014

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, ,
1Department of Chemistry and Biochemistry,University of Notre Dame, 2Harper Cancer Research Institute,University of Notre Dame

Chapters

  • 00:05Title
  • 01:28Three-dimensional Cell Culture and Preparation of Agarose Coated 96 Well Plates
  • 02:32Seed and Harvest the Three-dimensional Cultures and Embed in Gelatin
  • 04:11Slice and Thaw Mount the Gelatin-embedded Three-dimensional Cell Cultures for Mass Spectrometric Imaging
  • 05:03Matrix Application and Sample Preparation for MALDI Imaging
  • 05:42MALDI-MSI Analysis Using a MALDI-TOF System and Optional Data Analysis Methods
  • 07:28Results: Mass Spectrometry Imaging of Three-dimensional Cell Cultures
  • 07:49Conclusion

Summary

Automatic Translation

Automatic Translation

Immortaliserede kræft cellelinjer kan dyrkes som 3D-cellekulturer, en værdifuld model for biologisk forskning. Denne protokol beskriver massespektrometri billeddannelse af 3D cellekulturer, herunder forbedringer i prøveforberedelse platform. Målet med denne protokol er at instruere brugerne at forberede 3D cellekulturer til massespektrometri billeddannelse analyse.

Tags

Keywords: 3D Cell CultureMass Spectrometry ImagingMALDI-MSISample PreparationColon CancerSpheroidsTumor NodulesSpatial AnalysisMolecular Distribution

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