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对于外小泡的使用流式细胞仪分析技术

JoVE Journal
Biology

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JoVE Journal Biology

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

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对于外小泡的使用流式细胞仪分析技术

DOI:

10.3791/52484-v

•

09:39 min

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March 17, 2015

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Heather Inglis, Philip Norris^2,3, Ali Danesh³

¹Blood Systems Research Institute, ²Department of Medicine,University of California, San Francisco, ³Department of Laboratory Medicine,University of California, San Francisco

Chapters

00:05Title
01:23Individual Extracellular Vesicle Detection
05:33Bead-based Detection of EVs and Exosomes
07:19Results: Representative Individual and Bead-based Detection Data
08:49Conclusion

Summary

Automatic Translation

使用流式细胞仪（FCM）外囊泡（电动车）的测量许多不同的方法存在。确定最合适的方法时使用的几个方面应予以考虑。两个协议用于测量电动车使用的是单独的检测或珠为基础的方法介绍。

Tags

Extracellular Vesicles Flow Cytometry Cell-cell Communication Biological Processes Analysis Techniques EV Processing Bead-based Approach Individual Detection Method Signal-to-noise Ratio Background Fluorescence Clinical Samples Exosomes

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