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利用衣壳抗原掺入战略的腺病毒血清型5载体疫苗方法的发展
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
DOI:

13:36 min

May 06, 2015

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Chapters

  • 00:05Title
  • 02:31Construction of Modified Plasmid pAd5/H5-HVR1-KWAS-HVR5-His6
  • 05:09Preparation of Modified Ad5 Viral Vector Ad5/H5-HVR1-KWAS-HRV5-His6
  • 09:50Validation of the Rescued Viral Vector
  • 11:22Results: Evaluation of the Divalent Ad5 Vector Ad5/H5-HVR1-KWAS-HVR5-His6
  • 13:02Conclusion

Summary

Automatic Translation

在这里,我们提出了一个协议,以产生证明性的原则,二价5型腺病毒(Ad5的)载体的Ad5 / H5-HVR1-KWAS-HVR5-他6通过利用衣壳抗原掺入策略。此载体被证明表现出定性的健身,能力逃脱的Ad5阳性血清在体外 ,和抗原性以及免疫原性的结合抗原。

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