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Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology

DOI:
10.3791/52159-v

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08:27 min

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March 24, 2015

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, , , , , , , ,
1Experimmune, A Center for Immunotherapeutic Development, Department of Immunotherapeutics and Biotechnology,Texas Tech University Health Sciences Center, 2Resonant Sensors Incorporated, 3University of Texas Arlington

Chapters

  • 00:05Title
  • 02:13Addition of Biotinylated Antibody
  • 04:40Addition of Analyte
  • 07:03Results: FLSL/HLA Complexes are Detected in Patient Plasma Samples
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

Intact class I HLA/peptide complexes are shed by cancer cells, representing a potential relevant cancer biomarker. Utilizing label-free sensor technology and T-cell receptor mimicking monoclonal antibodies, detection of shed MIF/HLA-A*02:01 complexes in MDA-MB-231 cell supernatants, spiked human serum, and patient plasma is demonstrated, enabling development of a novel cancer diagnostic platform.

Tags

Breast CancerHuman Leukocyte Antigen (HLA)T-cell Receptor Mimic (TCRm) Monoclonal AntibodiesMacrophage Migration Inhibitory Factor (MIF)NY-ESO-1Label-free BiosensorGuided-mode Resonance (GMR) SensorCancer BiomarkersPersonalized MedicineCompanion DiagnosticsImmunotherapy

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