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Eine standardisierte Methode für die Analyse von Leber sinusoidalen Endothelzellen und ihre Fenestrationen durch Rasterelektronenmikroskopie
JoVE Journal
Biology
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JoVE Journal Biology
A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy
DOI:

08:38 min

April 30, 2015

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Chapters

  • 00:05Title
  • 01:07Perfusion Fixation of Liver
  • 03:32Specimen Mounting and Analysis
  • 05:29Calculations and Presentation of Data
  • 06:33Results: Scanning Electron Microscopy Analysis of Liver Sinusoidal Endothelial Cells
  • 08:12Conclusion

Summary

Automatic Translation

The fenestrated liver sinusoidal endothelial cell is a biologically important filter system that is highly influenced by various diseases, toxins, and physiological states. These changes significantly impact on liver function. We describe methods for the standardisation of the measurement of the size and number of fenestrations in these cells.

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