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无标记技术的单细胞分泌物的时空成像
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
DOI:

09:09 min

November 23, 2015

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Chapters

  • 00:05Title
  • 01:05Nanostructure Fabrication
  • 03:23Chip Cleaning and Application of Self-assembled Monolayer
  • 04:07LSPR General Settings – Chip Preparation and Mounting
  • 06:39LSPR Imaging of Anti-c-myc Secretions from 9E10 Hybridoma Cells
  • 07:14Results: Spatio-temporal Imaging of Single Cell Secretions by LSPRi
  • 08:43Conclusion

Summary

Automatic Translation

细胞间的通信为内和细胞外的控制各种生理活性的关键。本文描述了测量的单细胞的分泌物的时空性质的协议。为了实现这一目标,多学科的方法是使用集成了无标记纳米等离子体激感测与活细胞成像。

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