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Journal / Bioengineering / Measuring TCR-pMHC Binding In Situ using a…
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Bioengineering
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JoVE Journal Bioengineering
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
 

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Article DOI: 10.3791/53157-v • 19:06 min • October 30th, 2015
October 30th, 2015 •
1, 1, 2
1Institute for Applied Physics - Biophysics, Vienna University of Technology, 2Institute for Hygiene and Applied Immunology, Medical University of Vienna

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Summary
Automatic Translation

This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.

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Keywords: TCR-pMHC Binding FRET-based Microscopy T-cell Antigen Sensitivity Planar Lipid Bilayer Single Molecule FRET Receptor-ligand Interactions
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