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A Micropatterning Assay for Measuring Cell Chirality
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Bioengineering
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JoVE Journal Bioengineering
A Micropatterning Assay for Measuring Cell Chirality

A Micropatterning Assay for Measuring Cell Chirality

DOI:
10.3791/63105-v

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08:07 min

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March 11, 2022

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, , ,
1Department of Biomedical Engineering,Rensselaer Polytechnic Institute, 2Center for Biotechnology & Interdisciplinary Studies,Rensselaer Polytechnic Institute, 3Department of Biomedical Engineering,Columbia University, 4Department of Medicine,Columbia University, 5Department of Biological Sciences,Rensselaer Polytechnic Institute, 6Center for Modeling, Simulation, and Imaging in Medicine,Rensselaer Polytechnic Institute

Chapters

  • 00:04Introduction
  • 00:35Microcontact Printing
  • 03:36Seeding Cells onto Micropatterned Slides
  • 04:53Image Collection and Cell Chirality Characterization
  • 06:38Results: Effect of the Drug Treatment on the Cell Chirality
  • 07:28Conclusion

Summary

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We present a protocol for determining multicellular chirality in vitro, using the micropatterning technique. This assay allows for automatic quantification of the left-right biases of various types of cells and can be used for screening purposes.

Tags

MicropatterningCell ChiralityChiral MorphogenesisTitanium Gold-coated SlideOctadecanethiolPDMS StampsEG3 SolutionCell ResearchAutomated High-throughput Processing

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