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Bruke<em> Ex Vivo</em> Upright Droplet Kulturer av Whole føtale organer å studere utviklingsprosesser i løpet Mouse organogenesen
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
DOI:

09:47 min

October 21, 2015

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Chapters

  • 00:05Title
  • 01:57Isolation of Fetal Testes from Mus musculus
  • 04:45Culturing of Gonad with a Small Molecule Inhibitor
  • 07:01Results: Ex Vivo Droplet Culture of Fetal Whole Gonad-mesonephros Complexes
  • 08:32Conclusion

Summary

Automatic Translation

The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.

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