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Developmental Biology

转基因器官皮肤培养使用灭活人真皮代

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Developmental Biology

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JoVE Journal Developmental Biology

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

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转基因器官皮肤培养使用灭活人真皮代

DOI:

10.3791/53280-v

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09:16 min

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December 14, 2015

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Jingting Li², George L. Sen²

¹Department of Dermatology,UCSD Stem Cell Program, University of California, San Diego, ²Department of Cellular and Molecular Medicine,UCSD Stem Cell Program, University of California, San Diego

Chapters

00:05Title
00:50Preparation of Devitalized Human Dermis
02:21Genetic Modification of Primary Human Keratinocytes
04:33Building an Organotypic Cassette
05:09Organotypic Skin Cultures
07:03Results: Morphology and Gene Expression Analysis of Genetically Modified Human Epidermis
08:28Conclusion

Summary

Automatic Translation

The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.

转基因器官皮肤培养使用灭活人真皮代

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