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Recupero della fluorescenza dopo Fusione di una Droplet misurare la diffusione bidimensionale di un fosfolipide monostrato
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
DOI:

07:54 min

October 15, 2015

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Chapters

  • 00:05Title
  • 00:45Preparation of Phospholipid Monolayer at a Flat Air-water Interface
  • 02:32Preparation of Phospholipid Monolayer at the Curved Surface of a Droplet
  • 04:00Imaging Fluorescence Recovery after Droplet Merging
  • 05:17Results: Diffusion Coefficient of DOPC and DPPC Monolayer as a Function of Surface Pressure
  • 06:39Conclusion

Summary

Automatic Translation

We present a new technique to measure the lateral diffusion of a surface active species at the fluid-fluid interface by merging a droplet monolayer onto a flat monolayer.

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