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Chapters
- 00:05Title
- 00:58Generation and Expression of Fusion Proteins and Free Dam Proteins
- 03:12Library Preparation: Fragmentation
- 05:29Library Preparation: Size Selection
- 07:26Results: Detecting Lamin B1 Binding Regions on the Mouse Chromosome One
- 08:20Conclusion
Vi beskriver heri et assay ved kobling DNA methyltransferase adenin identifikation (DamID) til high throughput sekventering (DamID-seq). Denne forbedrede fremgangsmåde giver en højere opløsning og en større dynamikområde og muliggør analyse DamID-seq data i forbindelse med andre højt gennemløb sekventering data såsom chip-seq, RNA-seq, etc.
Tags
DamID-seqGenome-wide MappingProtein-DNA InteractionsHigh Throughput SequencingAdenine-methylated DNA FragmentsNuclear OrganizationTranscription RegulationMammalian CellsGenomic RegionsAmyloidTranscription FactorsHigh ResolutionDNA MicroarraysCross-analysisGene CloningDamID VectorLentiviral StocksLentiviral Expression SystemCotransfectionLentivirus Infection
