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Metoder til at studere Mrp4-holdige Makromolekylær Komplekser i forordningen af ​​fibroblast Migration
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
DOI:

10:43 min

May 19, 2016

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Chapters

  • 00:05Title
  • 01:20Preparation of Cells and Creating Wounds
  • 02:50Monitor Cell Migration and Data Analysis
  • 04:09Förster Resonance Enegry Transfer
  • 07:17Data Analysis
  • 08:34Results: MRP4 Regulation of Fibroblast Migration
  • 09:40Conclusion

Summary

Automatic Translation

MRP4 regulerer forskellige cykliske nukleotid-afhængig signalering arrangementer, herunder en nylig belyst rolle i celle migration. Vi beskriver en direkte, men flerstrenget tilgang at optrævle de nedstrøms molekylære mål for MRP4 resulterer i identifikation af et unikt MRP4 interactome der spiller centrale roller i finjusteret regulering af fibroblast migration.

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