/

Cancer Research

/

如何学习基底膜刚度生物物理触发在前列腺癌和其他与年龄相关的病理或代谢性疾病

JoVE Journal
Cancer Research

This content is Free Access.

JoVE Journal Cancer Research

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

Please note that all translations are automatically generated. Click here for the English version.

如何学习基底膜刚度生物物理触发在前列腺癌和其他与年龄相关的病理或代谢性疾病

DOI:

10.3791/54230-v

•

13:18 min

•

September 20, 2016

•

Mercedes Rodriguez-Teja, Claudia Breit, Mitchell Clarke, Kamil Talar, Kai Wang, Mohammad A. Mohammad, Sage Pickwell, Guillermina Etchandy, Graeme J. Stasiuk, Justin Sturge

¹Departamento de Genética, Facultad de Medicina,Universidad de la República (UDELAR), ²Department of Mechanistic Cell Biology,Max Planck Institute of Molecular Physiology, ³School of Biological, Biomedical & Environmental Sciences,University of Hull

Chapters

00:05Title
01:38Induction of Basement Membrane Stiffness Induced by GLA Treatment
04:18Photometric and SDS-PAGE Analysis of Reconstituted Basement Membrane
06:27Immunofluorescent Microscopy Analysis
09:093D Culture of Prostate Gland Acini and Prostate Tumor Cell Aggregates on Native and Stiff Reconstituted Basement Membrane
10:41Results: Prostate Cell Reactions to Changes in Basement Membrane Stiffness
12:02Conclusion

Summary

Automatic Translation

在这里，我们解释模型生物物理微环境，其中交联和提高晚期糖基化产物（AGEs）引起的基底膜（BM）的刚度病理意义的协议。

Tags

Keywords: Basement Membrane Stiffness Thickness Advanced Glycation Prostate Cancer Metastasis Age-related Pathologies Metabolic Diseases Cell Invasion 3D Cell Culture Cross-linking Biophysical Trigger

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克

超极化<sup> 13</sup>ç代谢磁共振波谱与成像

结肠-26癌荷瘤小鼠为癌症恶病质的研究模型

线粒体膜电位检测 CLIC4 击倒诱导 HN4 细胞凋亡的实验研究

磷酸浓缩结合无标签定量质谱法研究前列腺癌的 Phosphoproteome

酵母作为开发功能分析研究人类P53的机箱

使用小鼠化疗引起的粘炎模型评估小肠损伤和适应的重要端点和增殖标记

体外测定研究肿瘤-巨噬菌体相互作用

前列腺有机体培养作为工具，将基因型和突变谱转化为药理反应

利用活体中鸡胆膜研究妇科和泌尿癌

Read Article