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Como estudiar membrana basal para la rigidez como un disparador de Biofísica en el cáncer de próstata y otras patologías relacionadas con la edad o enfermedades metabólicas
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
DOI:

13:18 min

September 20, 2016

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Chapters

  • 00:05Title
  • 01:38Induction of Basement Membrane Stiffness Induced by GLA Treatment
  • 04:18Photometric and SDS-PAGE Analysis of Reconstituted Basement Membrane
  • 06:27Immunofluorescent Microscopy Analysis
  • 09:093D Culture of Prostate Gland Acini and Prostate Tumor Cell Aggregates on Native and Stiff Reconstituted Basement Membrane
  • 10:41Results: Prostate Cell Reactions to Changes in Basement Membrane Stiffness
  • 12:02Conclusion

Summary

Automatic Translation

Aquí se explica un protocolo para modelar el microambiente biofísico donde la reticulación y el aumento de la rigidez de la membrana basal (BM) inducida por productos finales de glicación avanzada (AGEs) tiene relevancia patológica.

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