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Prostat Kanseri ve Diğer Yaşa bağlı Patolojilerde veya Metabolik Hastalıklarda bir Biyofiziksel Tetik olarak Bazal Membran Rijitliğinin Eğitim Nasıl
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
DOI:

13:18 min

September 20, 2016

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Chapters

  • 00:05Title
  • 01:38Induction of Basement Membrane Stiffness Induced by GLA Treatment
  • 04:18Photometric and SDS-PAGE Analysis of Reconstituted Basement Membrane
  • 06:27Immunofluorescent Microscopy Analysis
  • 09:093D Culture of Prostate Gland Acini and Prostate Tumor Cell Aggregates on Native and Stiff Reconstituted Basement Membrane
  • 10:41Results: Prostate Cell Reactions to Changes in Basement Membrane Stiffness
  • 12:02Conclusion

Summary

Automatic Translation

Burada çapraz ve ileri glikasyon nihai ürünlerin (AGE) tarafından uyarılan bazal membran (BM) artan sertlik patolojik ilgisi biyofiziksel mikro modelleme için bir protokol açıklar.

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