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JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
DOI:

08:53 min

August 31, 2016

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Chapters

  • 00:05Title
  • 01:12Donor and Competitor Bone Marrow Cell Preparation
  • 02:58Donor Hematopoietic Stem Cell (HSC) Equivalent Identification
  • 04:34Bone Marrow Transplants
  • 05:27Peripheral Blood Analysis
  • 06:39Results: Representative Peripheral Blood Chimerism
  • 07:53Conclusion

Summary

Automatic Translation

This protocol provides step-by-step guidelines for setting up competitive mouse bone marrow transplant experiments to study hematopoietic stem/progenitor cell function without prior purification of stem cells by cell sorting.

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