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Transkripsjonell Analyse av begynnende RNA FISH av<em> I Vivo</em> Trophoblast? Trophoblast kjempeceller eller<em> In Vitro</em> Kortsiktige Kulturer av Ectoplacental Cone explants
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Chapters
- 00:05Title
- 00:58Preparing Secondary Trophoblast Giant Cells (TGCs)
- 02:11Fixation and Permeabilization
- 02:50RNA FISH: Part 1 (Probe Hybridization)
- 04:29RNA FISH: Part 2 (Post-hybridization Washes)
- 05:43Results: Probed TGCs
- 07:15Conclusion
Trophoblast giant cells (TGCs) play a key role in the placenta to ensure a healthy pregnancy. We present a protocol for assessing the transcriptional status of genes in TGCs by nascent fluorescent in situ hybridization on cryostat sections of post-implantation embryos or short-term cultures of embryonic day 7 ectoplacental cones.
Tags
Transcriptional AnalysisNascent RNA FISHTrophoblast Giant CellsEctoplacental Cone ExplantsIn VivoIn VitroSingle Cell AnalysisGene ExpressionX Chromosome InactivationImprintingEmbryo DissectionEPC IsolationCell CultureCell FixationCell PermeabilizationFluorescent Probe PrecipitationEthanol Precipitation
