Journal
/
/
항원 중심의 성 대장염 모델의 개발은 항원이 T 세포에 세포를 제시하여 항원의 프리젠 테이션을 공부하기
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
DOI:

06:57 min

September 18, 2016

, , ,

Chapters

  • 00:05Title
  • 01:28Colonic Lamina propria Cell Isolation
  • 03:58Results: Host CX3CR1+ Macrophages Communicate with Donor OT-II Cells During OVA-driven Transfer Colitis
  • 06:03Conclusion

Summary

Automatic Translation

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

Related Videos

Read Article