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Immunology and Infection
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通过流式细胞仪大鼠肾脏巨噬细胞的表型特征
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Immunology and Infection
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
通过流式细胞仪大鼠肾脏巨噬细胞的表型特征
DOI:
10.3791/54599-v
•
07:14 min
•
October 18, 2016
•
Alfonso Rubio-Navarro
,
Melania Guerrero-Hue
,
Beatriz Martín-Fernandez
,
Isabel Cortegano
,
Elena Olivares-Alvaro
,
Natalia de las Heras
,
Mario Alía
,
Belén de Andrés
,
María Luisa Gaspar
,
Jesús Egido
,
Juan Antonio Moreno
1
Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz
,
Autonoma University
,
2
Department of Physiology, Faculty of Medicine
,
Complutense University
,
3
Department of Immunology, Centro Nacional de Microbiologìa
,
Instituto de Salud Carlos III (ISCIII)
Chapters
00:05
Title
00:51
Kidney Perfusion and Extraction
02:07
Kidney Digestion and Single Cell Suspension
03:13
Cell Staining and Flow Cytometry
04:29
Results: Representative Rat Peritoneal Macrophage Characterization
05:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
该原稿描述的表型,并通过流式细胞从大鼠肾脏定居巨噬细胞的定量分析的详细协议。所得染色的细胞也可以用于其它应用,包括细胞分选,基因表达分析或功能性研究,从而增加在实验模型中获得的信息。
Tags
Macrophages
Kidney
Flow Cytometry
Characterization
Phenotype
M1
M2
Inflammation
Renal Disease
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