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通过流式细胞仪大鼠肾脏巨噬细胞的表型特征
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Immunology and Infection
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JoVE Journal Immunology and Infection
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
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通过流式细胞仪大鼠肾脏巨噬细胞的表型特征

DOI:
10.3791/54599-v

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07:14 min

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October 18, 2016

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, , , , , , , , , ,
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz,Autonoma University, 2Department of Physiology, Faculty of Medicine,Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa,Instituto de Salud Carlos III (ISCIII)

Chapters

  • 00:05Title
  • 00:51Kidney Perfusion and Extraction
  • 02:07Kidney Digestion and Single Cell Suspension
  • 03:13Cell Staining and Flow Cytometry
  • 04:29Results: Representative Rat Peritoneal Macrophage Characterization
  • 05:53Conclusion

Summary

Automatic Translation

Automatic Translation

该原稿描述的表型，并通过流式细胞从大鼠肾脏定居巨噬细胞的定量分析的详细协议。所得染色的细胞也可以用于其它应用，包括细胞分选，基因表达分析或功能性研究，从而增加在实验模型中获得的信息。

Tags

MacrophagesKidneyFlow CytometryCharacterizationPhenotypeM1M2InflammationRenal Disease

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