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发电<em>德诺</em>抗原特异性人T细胞受体通过中心的Hemichain的逆转录病毒转导
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
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发电<em>德诺</em>抗原特异性人T细胞受体通过中心的Hemichain的逆转录病毒转导

DOI:
10.3791/54697-v

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08:48 min

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October 25, 2016

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, , , , , , , , ,
1Department of Immunology,University of Toronto, 2Princess Margaret Cancer Centre,University Health Network

Chapters

  • 00:05Title
  • 00:35T Cell Receptor (TCR) Hemichain-encoded Retroviral Construct Preparation
  • 01:41Stable Packaging Cell Line Generation
  • 03:08Human T Cell Activation and Transduction
  • 05:04Novel Antigen-specific TCR Clone Reconstitution
  • 06:25Results: Centric TCR Hemichains Pair with Different Counter-chains to Form Antigen-specific TCRs with Varying Affinity
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

这里我们描述了一种新方法，以产生抗原特异性T细胞受体（TCR）通过配对现有的TCR的TCRα或TCRβ，具有感兴趣的抗原特异性，与外周T细胞受体库的补充hemichain。 从头产生的TCR保留抗原特异性有不同的亲和力。

Tags

Keywords: De Novo T Cell ReceptorTCR Hemi-chainRetroviral TransductionAntigen-specific T CellsGene EngineeringImmunotherapyPCR CloningBacterial TransformationPlasmid Purification293GPG Cell TransfectionPG-13 Packaging Cell LinePBMC Activation

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