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瞬时转染293T细胞复制缺陷型逆转录病毒生产
JoVE Journal
Biology
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JoVE Journal Biology
Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
DOI:

09:40 min

December 04, 2007

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Chapters

  • 00:08Introduction
  • 00:51Preparation of 293T cells
  • 03:51Preparation of transfection mixture
  • 04:52Transfection
  • 07:02Collection of virus
  • 08:00Discussion

Summary

Automatic Translation

这种技术演示了一个有效的方式来准备复制缺陷型逆转录病毒编码的人类癌基因的股票,随后诱导骨髓增殖性疾病的小鼠模型中使用。

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