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Biology
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瞬时转染293T细胞复制缺陷型逆转录病毒生产
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Biology
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JoVE Journal
Biology
Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
Please note that all translations are automatically generated.
Click here for the English version.
瞬时转染293T细胞复制缺陷型逆转录病毒生产
DOI:
10.3791/550-v
•
09:40 min
•
December 04, 2007
•
L Cristina Gavrilescu
,
Richard A Van Etten
1
Molecular Oncology Research Institute
,
Tufts University
Chapters
00:08
Introduction
00:51
Preparation of 293T cells
03:51
Preparation of transfection mixture
04:52
Transfection
07:02
Collection of virus
08:00
Discussion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种技术演示了一个有效的方式来准备复制缺陷型逆转录病毒编码的人类癌基因的股票,随后诱导骨髓增殖性疾病的小鼠模型中使用。
Tags
Replication-defective Retrovirus
Transient Transfection
293T Cells
Human Myeloproliferative Diseases
Oncogenes
Bcr-Abl
Growth Factor Receptor-derived Oncogenes
ZNF198-FGFR1
Bcr-PDGFRα
Mouse Model
Bone Marrow Cells
Retroviral Vector
Gag-pol-env Packaging Functions
Calcium Phosphate Transfection
Chloroquine
Ecotropic Virus
Culture Supernatant
Cell-lines
Transformation Studies
In Vitro Studies
Primary Cells
Mouse Model
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