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Chapters
- 00:08Introduction
- 00:51Preparation of 293T cells
- 03:51Preparation of transfection mixture
- 04:52Transfection
- 07:02Collection of virus
- 08:00Discussion
Tags
Replication-defective RetrovirusTransient Transfection293T CellsHuman Myeloproliferative DiseasesOncogenesBcr-AblGrowth Factor Receptor-derived OncogenesZNF198-FGFR1Bcr-PDGFRαMouse ModelBone Marrow CellsRetroviral VectorGag-pol-env Packaging FunctionsCalcium Phosphate TransfectionChloroquineEcotropic VirusCulture SupernatantCell-linesTransformation StudiesIn Vitro StudiesPrimary CellsMouse Model
