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Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
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Neuroscience
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JoVE Journal Neuroscience
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors

Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors

DOI:
10.3791/55045-v

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05:18 min

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January 21, 2017

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, , , ,
1Parkinson’s Institute and Clinical Center

Chapters

  • 00:00Title
  • 00:51Transferring Meninges to Cutting Dish
  • 01:14Washing Meninges to Remove Blood
  • 01:52Sectioning Meninges under Dissecting Microscope
  • 02:49Transferring Meninges to Culture Plate
  • 03:19Adding Coverslips over Meninges
  • 04:20Representative Results
  • 04:50Conclusion

Summary

Automatic Translation

Automatic Translation

The leptomeninges explant culture protocol from human postmortem brain is a technically robust and simple way to derive fibronectin-positive meningeal fibroblasts within 6-8 weeks and cryopreserve approximately 20-30 million cells.

Tags

Keywords: LeptomeningesExplant CulturePostmortemHuman BrainParkinson’s DiseaseFibroblastCell-based AssayLewy Body PathologyPhosphate-buffered SalineDissecting MicroscopeGelatinMeninges Media

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