Journal
/
/
Dérivation des cultures leptoméninge explants de Postmortem humaines donateurs cerveau
JoVE Journal
Neuroscience
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
DOI:

05:18 min

January 21, 2017

, , , ,

Chapters

  • 00:00Title
  • 00:51Transferring Meninges to Cutting Dish
  • 01:14Washing Meninges to Remove Blood
  • 01:52Sectioning Meninges under Dissecting Microscope
  • 02:49Transferring Meninges to Culture Plate
  • 03:19Adding Coverslips over Meninges
  • 04:20Representative Results
  • 04:50Conclusion

Summary

Automatic Translation

Le protocole de culture leptoméninge explant de post-mortem du cerveau humain est un moyen techniquement robuste et simple pour obtenir des fibroblastes méningées fibronectine positifs dans les 6-8 semaines et cryoconservation environ 20-30000000 cellules.

Related Videos

Read Article