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Neuroscience
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死後ヒト脳ドナーから軟膜植片培養物の導出
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Neuroscience
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Neuroscience
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
Please note that all translations are automatically generated.
Click here for the English version.
死後ヒト脳ドナーから軟膜植片培養物の導出
DOI:
10.3791/55045-v
•
05:18 min
•
January 21, 2017
•
Kelsey Lee*
1
,
Ou Chong Saetern*
1
,
Andrew Nguyen
,
Leonardo Rodriguez
,
Birgitt Schüle
1
Parkinson’s Institute and Clinical Center
Chapters
00:00
Title
00:51
Transferring Meninges to Cutting Dish
01:14
Washing Meninges to Remove Blood
01:52
Sectioning Meninges under Dissecting Microscope
02:49
Transferring Meninges to Culture Plate
03:19
Adding Coverslips over Meninges
04:20
Representative Results
04:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
人間の死後脳から軟膜外植片培養プロトコルは6-8週間以内にフィブロネクチン陽性髄膜線維芽細胞を導出し、約20から30000000細胞を凍結保存するための技術的に堅牢かつ簡単な方法です。
Tags
Leptomeninges
Explant Culture
Postmortem
Human Brain
Parkinson’s Disease
Fibroblast
Cell-based Assay
Lewy Body Pathology
Phosphate-buffered Saline
Dissecting Microscope
Gelatin
Meninges Media
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