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Dextran aumenta a eficiência de Lentivirus transdução das células NK primário murino e humano
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1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute,The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute,The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute,The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute,The Blood Center of Wisconsin, 5Department of Microbiology and Immunology,The Medical College of Wisconsin, 6Department of Pediatrics,The Medical College of Wisconsin, 7Department of Medicine,The Medical College of Wisconsin
Chapters
- 00:05Title
- 00:44Purification and Expansion of Murine Primary NK Cells
- 02:03Purification and Expansion of Human Primary NK Cells
- 03:23Transduction of Murine and Human Primary NK Cells with Lentivirus
- 06:25Results: Lentiviral Transduction Efficiency Enhanced with Dextran
- 07:58Conclusion
O objetivo deste estudo foi a formulação de tecnologias que permitem a transdução de gene bem sucedido na primária natural killer (NK) as células. O dextran-mediada Lentivirus transdução de humanos ou rato primário NK células os resultados, em maior eficiência de expressão do gene. Este método de transdução gene vastamente irá melhorar a manipulação genética de células NK.
