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可调水凝胶由肺细胞外基质的三维细胞培养

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

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可调水凝胶由肺细胞外基质的三维细胞培养

DOI:

10.3791/55094-v

•

10:54 min

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January 17, 2017

•

Patrick A. Link, Robert A. Pouliot, Nabil S. Mikhaiel, Bethany M. Young, Rebecca L. Heise²

¹Department of Biomedical Engineering,Virginia Commonwealth University, ²Department of Physiology and Biophysics,Virginia Commonwealth University

Chapters

00:05Title
00:52Hydrogel Formation
03:45Lung Processing and Micro-porous Gel Formation
06:55Culturing of Micro-porous Gels
08:53Results: Hydrogel Analysis
10:13Conclusion

Summary

Automatic Translation

这是创建从肺的细胞外基质的三维细胞培养物的支架的方法。完好肺被加工成可支持细胞以三维生长的水凝胶。

Tags

Keywords: Tunable Hydrogels Pulmonary Extracellular Matrix 3D Cell Culture Lung ECM Decellularization Porcine Lung Triton X-100 Deoxycholate Sodium Chloride DNase Respiratory Zones

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