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通过“Acufection”和它的皮肤研究中的应用的经济型DNA递送系统的开发
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Development of an Economical DNA Delivery System by “Acufection” and its Application to Skin Research
DOI:

09:42 min

April 19, 2017

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Chapters

  • 00:05Title
  • 00:40Large Scale Preparation of Endotoxin-free Plasmid DNA
  • 01:36Acufection Procedure and Post-operative Care
  • 03:17Imiquimod Treatment of IL-15ΔE7-acufected Skin
  • 04:06Mouse Skin Homogenation
  • 05:20Immunohistochemical Staining of the Skin Section
  • 07:23Results: Acufection Enables an Efficient Gene Trasfer While Inducing Minimal Skin Damage
  • 08:37Conclusion

Summary

Automatic Translation

这个协议是用于在小鼠皮肤中表达裸质粒DNA具有成本效益的选择。该协议的总体目标是提供免疫相关基因植入皮肤组织划定的皮肤炎症特定基因的功能作用。

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