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Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
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Developmental Biology
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JoVE Journal Developmental Biology
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

DOI:
10.3791/55243-v

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10:15 min

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March 22, 2017

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, , ,
1Department of Pediatrics,UConn Health, 2Department of Gastroenterology,Connecticut Children’s Medical Center, 3Department of Surgery,Connecticut Children’s Medical Center

Chapters

  • 00:05Title
  • 01:213T3 Feeder Cell Culture and Irradiation
  • 02:31Pediatric Esophageal Biospy Acquisition and Processing
  • 05:00Cell Culture Expansion, Freezing, and Storage
  • 07:17Results: Representative Human Esophageal Cell Characterization
  • 08:59Conclusion

Summary

Automatic Translation

Automatic Translation

Expansion of human pediatric esophageal epithelial cells utilizing conditional reprogramming provides investigators with a patient-specific population of cells that can be utilized for engineering esophageal constructs for autologous implantation to treat defects or injury and serve as a reservoir for therapeutic screening assays.

Tags

Keywords: Conditional ReprogrammingEsophageal Epithelial CellsDisease InvestigationEosinophilic EsophagitisEsophageal Atresia3D ScaffoldPatient-specificEndoscopic BiopsyFeeder Cells3T3 CellsIrradiationConditional Programming MediumSerum-free Keratinocyte MediumPrimocinFormalinCryomold

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