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小儿食管上皮细胞重编程的条件在组织工程和疾病使用调查

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Developmental Biology

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JoVE Journal Developmental Biology

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

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小儿食管上皮细胞重编程的条件在组织工程和疾病使用调查

DOI:

10.3791/55243-v

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10:15 min

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March 22, 2017

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Todd J. Jensen, Christopher Foster, Wael Sayej, Christine M. Finck

¹Department of Pediatrics,UConn Health, ²Department of Gastroenterology,Connecticut Children’s Medical Center, ³Department of Surgery,Connecticut Children’s Medical Center

Chapters

00:05Title
01:213T3 Feeder Cell Culture and Irradiation
02:31Pediatric Esophageal Biospy Acquisition and Processing
05:00Cell Culture Expansion, Freezing, and Storage
07:17Results: Representative Human Esophageal Cell Characterization
08:59Conclusion

Summary

Automatic Translation

利用条件重新编程人类小儿食管上皮细胞的扩张提供了可用于工程食管构造为自体移植来治疗缺损或损伤，并作为治疗性筛选测定的储存器单元的特定病人群调查。

Tags

Keywords: Conditional Reprogramming Esophageal Epithelial Cells Disease Investigation Eosinophilic Esophagitis Esophageal Atresia 3D Scaffold Patient-specific Endoscopic Biopsy Feeder Cells 3T3 Cells Irradiation Conditional Programming Medium Serum-free Keratinocyte Medium Primocin Formalin Cryomold

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