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Betinget Omprogrammering af Pediatric Menneskelig Esophageal epitelceller til brug i Tissue Engineering og Sygdom Investigation
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
DOI:

10:15 min

March 22, 2017

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Chapters

  • 00:05Title
  • 01:213T3 Feeder Cell Culture and Irradiation
  • 02:31Pediatric Esophageal Biospy Acquisition and Processing
  • 05:00Cell Culture Expansion, Freezing, and Storage
  • 07:17Results: Representative Human Esophageal Cell Characterization
  • 08:59Conclusion

Summary

Automatic Translation

Udvidelse af humane pædiatriske øsofageale epitelceller anvender betinget omprogrammering giver undersøgere med en patient-specifik population af celler, der kan anvendes til manipulering esophageal konstruktioner til autolog implantation til behandling defekter eller skade og tjene som et reservoir til terapeutiske screeningsassays.

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