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生物相容的液晶弹性体泡沫的合成中用作细胞支架的三维空间细胞培养

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

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生物相容的液晶弹性体泡沫的合成中用作细胞支架的三维空间细胞培养

DOI:

10.3791/55452-v

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13:38 min

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April 11, 2017

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Marianne E. Prévôt, Senay Ustunel, Leah E. Bergquist, Richard Cukelj, Yunxiang Gao, Taizo Mori, Lindsay Pauline, Robert J. Clements, Elda Hegmann

¹Liquid Crystal Institute,Kent State University, ²Chemical Physics Interdisciplinary Program, Liquid Crystal Institute,Kent State University, ³Department of Biological Sciences,Kent State University

Chapters

00:05Title
01:07Synthesis of α-Three-arm Star Block Copolymer (SBC-αCl)
04:37Fabrication of a Porous 3D Liquid Crystal Elastomer (LCE) Scaffold with Hexamethylene Diisocyanate (HDI) as a Crosslinker
07:27Seeding the LCE Scaffold Using Sterile Techniques and Imaging the LCE Construct
10:15Results: 3D Spatial Cell Cultures Prepared on LCE Foams
12:03Conclusion

Summary

Automatic Translation

这项研究提出了一种方法来制备3D，基于生物相容的侧链液晶弹性体（LCES）可生物降解的，泡沫状细胞支架。共聚焦显微镜实验表明，泡沫状LCES允许细胞附着，增殖和C2C12s成肌细胞的自发排列。

Tags

Keywords: Biocompatible Liquid Crystal Elastomer Foam Cell Scaffold 3D Cell Culture Biodegradable PFOTES Epsilon-caprolactone Alpha-chloro-epsilon-caprolactone Glycerol DL-lactide 2-ethylhexanoate Polymerization Vacuum Thermal Treatment

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