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In Vitro Imaging en kwantificering van de Drug Targeting Efficiency van fluorescent gelabelde GnRH analogen
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
DOI:

10:36 min

March 21, 2017

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Chapters

  • 00:05Title
  • 01:07GnRH Uptake
  • 02:39Type I GnRH Receptor Expression
  • 05:39Fluorescence Microscopy Imaging
  • 06:26FACS-assisted GnRH Quantification
  • 08:00Results: GnRH I Uptake and Type I GnRH Receptor Expression in Pharynx Carcinoma Cells
  • 09:25Conclusion

Summary

Automatic Translation

Selectief gemerkte fluorescerende GnRH-I, -II en -III derivaten betrouwbare middelen voor het opsporen en kwantificeren van de cellulaire opname. Dit manuscript introduceert experimenten te visualiseren, kwantificeren en te vergelijken de opname efficiëntie van deze GnRH conjugaten in diverse cellijnen.

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