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The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections
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Immunology and Infection
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JoVE Journal Immunology and Infection
The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

DOI:
10.3791/55532-v

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06:57 min

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May 08, 2017

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, , , , , ,
1Skaggs School of Pharmacy and Pharmaceutical Sciences,University of California, San Diego, 2Department of Mechanical and Aerospace Engineering,University of California, San Diego

Chapters

  • 00:05Title
  • 00:38Preparing Medium and Artificial Sputum for Capillary Tube Loading
  • 02:29Filling the WinCF Capillary Tubes with Inoculated Artificial Sputum Medium
  • 04:12Incubation and Imaging the Extraction
  • 05:38Results: Microbiological Growth in the WinCF Capillary Tubes
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

The mucus plugged airways of cystic fibrosis (CF) patients are an ideal environment for microbial pathogens to thrive. The manuscript describes a novel method for studying the CF lung microbiome in an environment that mimics where they cause disease and how alterations of chemical conditions can drive microbial dynamics.

Tags

Keywords: WinCF ModelArtificial Sputum MediumCystic Fibrosis LungAirway MicrobiologyBacterial PathogensMicrobial ResponsesMucus Plugged BronchioleLung InfectionsCapillary TubesHomogenized SputumIncubation TubesSterile Biohood

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