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多肽和蛋白质量化使用自动免疫-MALDI (iMALDI)
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
DOI:

08:57 min

August 18, 2017

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Chapters

  • 00:05Title
  • 01:06Generation of Ang I in Human Plasma
  • 03:01Immuno-enrichment of Ang I Using Antibody-coated Beads
  • 06:04Transfer of Beads onto MALDI Target Plate and MALDI-TOF-MS Analysis
  • 06:55Results: Quantification of Ang I Using iMALDI
  • 07:53Conclusion

Summary

Automatic Translation

提出了在使用自动的免疫-MALDI (iMALDI) 技术的复杂生物体液蛋白质定量的协议。

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