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Chemistry
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ペプチドおよびタンパク質定量を使用して自動免疫-MALDI (iMALDI)
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JoVE Journal
Chemistry
Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
Please note that all translations are automatically generated.
Click here for the English version.
ペプチドおよびタンパク質定量を使用して自動免疫-MALDI (iMALDI)
DOI:
10.3791/55933-v
•
08:57 min
•
August 18, 2017
•
Huiyan Li
,
Robert Popp
,
Bjorn Frohlich
,
Michael X. Chen
,
Christoph H. Borchers
3,4,5
1
University of Victoria-Genome BC Proteomics Centre
,
2
Jewish General Hospital
,
McGill University
,
3
Dept of Biochemistry and Microbiology
,
University of Victoria
,
4
Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital
,
McGill University
,
5
Gerald Bronfman Department of Oncology
,
Jewish General Hospital
Chapters
00:05
Title
01:06
Generation of Ang I in Human Plasma
03:01
Immuno-enrichment of Ang I Using Antibody-coated Beads
06:04
Transfer of Beads onto MALDI Target Plate and MALDI-TOF-MS Analysis
06:55
Results: Quantification of Ang I Using iMALDI
07:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
自動免疫 MALDI (iMALDI) 技術を使用して複雑な体液でプロテインの定量のためのプロトコルが表示されます。
Tags
Peptide Quantification
Protein Quantification
Immuno-MALDI
Automated Assay
Plasma Samples
Angiotensin I
Antibody Conjugation
Protein Biomarkers
Quantitative Proteomics
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