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Peptide et protéine Quantification moyen automatisé Immuno-MALDI (iMALDI)
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
DOI:

08:57 min

August 18, 2017

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Chapters

  • 00:05Title
  • 01:06Generation of Ang I in Human Plasma
  • 03:01Immuno-enrichment of Ang I Using Antibody-coated Beads
  • 06:04Transfer of Beads onto MALDI Target Plate and MALDI-TOF-MS Analysis
  • 06:55Results: Quantification of Ang I Using iMALDI
  • 07:53Conclusion

Summary

Automatic Translation

Un protocole pour la quantification de la protéine dans les liquides biologiques complexes à l’aide de la technologie automatisée immuno-MALDI (iMALDI) est présenté.

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