Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

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Biology

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Biology
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JoVE Journal Biology

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

DOI:

10.3791/56118-v

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13:32 min

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March 04, 2018

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Zoran Marinović, Jelena Lujić, Eszter Kása, Zsolt Csenki, Béla Urbányi, Ákos Horváth

¹Department of Aquaculture,Szent István University

Chapters

00:00Title
01:14Testis collection
03:29Ultra-rapid cooling of the testicular tissue
06:11Warming procedure
07:56Tissue digestion
10:27Viability evaluation and cell counting
11:28Representative Results
12:20Conclusions

Summary

Automatic Translation

The main purpose of this study was to adapt the needle immersed vitrification (NIV) procedure to cryopreserve whole zebrafish testes. Additionally, the repeatability of the method in five different zebrafish strains was tested.

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

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