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Zebrafish Spermatogonia 전체 Testes 바늘의 cryopreservation 몰입 울트라-빠른 냉각
JoVE Journal
Biology
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JoVE Journal Biology
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
DOI:

13:32 min

March 04, 2018

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Chapters

  • 00:00Title
  • 01:14Testis collection
  • 03:29Ultra-rapid cooling of the testicular tissue
  • 06:11Warming procedure
  • 07:56Tissue digestion
  • 10:27Viability evaluation and cell counting
  • 11:28Representative Results
  • 12:20Conclusions

Summary

Automatic Translation

이 연구의 주요 목적은 바늘에 맞게 vitrification (NIV) 절차 전체 zebrafish testes cryopreserve에 몰입 했다. 또한, 5 개의 다른 zebrafish 긴장에서 방법의 반복성 시험 되었다.

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